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. Author manuscript; available in PMC: 2024 Apr 2.

Published in final edited form as: J Vis Exp. 2023 Mar 17;(193):10.3791/65033. doi: 10.3791/65033

Table 7:

Primer sequences and targeted qPCR cycling conditions for the quality check of the ATAC-seq library.

Gene	Sequences (Forward)	Sequences (Reverse)
Negative control 1	TGCAAATGGCAAAATAGCTCGT	CCACACGTCCAGTGATTTGC
Negative control 2	TACCAATGTCCACCCTCTGA	TGCTTACACCTCAGCAGGAC
Adipoq	TCACTTCTAGTCGCTAAGCAAG	TCAATACTTTGCTGGGTCTGG
Fabp4	GGTCACATCTCAGAATCTCAGG	ACAGGCAAAGGTTAGAAGGATG
Plin1	CTAGGATGTGGGTGAAAGGTG	TCAAGACCTCTGCTCTCCTG
Pnpla2	GTAGGTGATGGTTGAAGTAGGTC	TGAGTCACTGTGCCTAACATG
Ucp1	GCCCTTGGATTGGCTGAAGA	CCACATGCTCAGGACACCAT

Steps	Temperature	Time	Cycles
Hold Stage	50°C	2 min	1
Hold Stage	95°C	10 min	1
PCR Stage	95°C	15 s	40
PCR Stage	60°C	1 min	40
Melt Curve Stage	95°C	15 s	1
	60°C	1 min
	95°C	15 s