Table 8:
Analysis of the qPCR results for the quality check.
| Raw Ct values | ||||||
|---|---|---|---|---|---|---|
| NC1 | NC2 | Adipoq | Fabp4 | Plin1 | Pnpla2 | |
| eWAT 1 | 28.97360516 | 29.1767311 | 21.8443975 | 23.8591576 | 25.0069361 | 21.7385616 |
| eWAT 2 | 29.01209068 | 28.9158125 | 22.0090828 | 24.0115328 | 25.2831001 | 22.0457344 |
| iWAT 1 | 30.67526627 | 30.9066448 | 21.9058771 | 24.0909586 | 25.1765642 | 21.9324293 |
| iWAT 2 | 29.73054695 | 30.0138845 | 22.1776199 | 24.9327497 | 26.8281002 | 23.2821865 |
| BAT 1 | 31.03091812 | 31.4215002 | 23.4410648 | 25.7339334 | 27.1350803 | 23.389307 |
| BAT 2 | 31.88665962 | 32.3051062 | 24.0364552 | 26.9744196 | 28.2373838 | 23.8191948 |
| Caculation of the enrichment of the targeted regions over negative control (NC) 1 | ||||||
| : 2 ^ -[ Ct (gene of interest) – Ct (negative control 1) ] | ||||||
| NC1 | NC2 | Adipoq | Fabp4 | Plin1 | Pnpla2 | |
| eWAT 1 | 1 | 0.86866634 | 139.992683 | 34.6419346 | 15.6345857 | 150.648607 |
| eWAT 2 | 1 | 1.06901211 | 128.267147 | 32.012377 | 13.2598317 | 125.049569 |
| iWAT 1 | 1 | 0.85182055 | 436.364266 | 95.9564361 | 45.2141378 | 428.406617 |
| iWAT 2 | 1 | 0.82168789 | 187.783568 | 27.8151157 | 7.47693373 | 87.3272766 |
| BAT 1 | 1 | 0.76282177 | 192.651991 | 39.3143657 | 14.8855207 | 199.689006 |
| BAT 2 | 1 | 0.74822987 | 230.75282 | 30.1114448 | 12.5470455 | 268.255665 |