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In vitro CKII phosphorylation of a GST-gB C-terminal tail chimeric protein. A GST-gBwt or gBAla C-terminal tail chimera was attached to microbeads and treated with either CKII or CKI and [32P]orthophosphate followed by magnetic bead purification and SDS-PAGE. (A) gBwt but not gBAla was phosphorylated by CKII. However, neither of the chimeric proteins was phosphorylated by CKI. (B) Both GST-gBwt and GST-gBAla, were stably expressed as determined by analysis by denaturing SDS-PAGE.
