FIG. 5.
The steady-state cell surface expression of gBAsp is greater than that of gBwt and gBAla on the PM of U373 cells. U373 cells were infected with RVV gBwt, RVV gBAla, or RVV gBAsp and then subjected to surface biotinylation to analyze differences in gB PM expression. Specifically, 35S-labeled U373 cells infected with RVV gBwt, RVV gBAla, or RVV gBAsp were pulsed with NHS-SS-biotin and the immunoprecipitated surface gB was analyzed by SDS-PAGE. This figure demonstrates that substantially more gBAsp than gBAla is detected at the surface of U373 cells. These results support the hypothesis that a charge at position 900 in the gB cytoplasmic tail increases surface expression in U373 cells. c-term, C terminus.