Figure 1.

Effect of HDAC inhibitors on inactive vitamin D₃-mediated induction of LL-37 gene expression in oral keratinocytes. OKF6/TERT-1 cells were incubated with either 0.1% Ethanol (EtOH) in the presence or absence of 1 µm inactive vitamin D3 for 24 h. Added to each was HDAC inhibitors at the following concentrations: PBA (1 mm); MS-275 (2 mm); Apidacin (1 µm); Ellagic acid (2 mm); Valproic acid (100 µm); and Nicotinamide (2 mm). Total mRNA was isolated and relative LL-37 mRNA levels were quantified by QRT-PCR relative to β-actin. Data are shown as mean (n = 3) ±SD. *p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.00005 by 2-way ANOVA and Tukey's post-hoc test.