TABLE 1.
Set of spiked-in standards added on raw supernatant to quantify the concentration step on protein binding with StrataClean resina
| Protein | MWb (kDa) | pIb | Gravy index |
|---|---|---|---|
| α-Lactalbumin | 14.3 | 4.4 | −0.169 |
| Glyceraldehyde-3-phosphate dehydrogenase | 37.0 | 3.5 | −0.053 |
| Alcohol dehydrogenase | 36.8 | 5.6 | 0.030 |
| Soybean trypsin inhibitor | 20.0 | 4.7 | −0.241 |
| Lysozyme | 14.30 | 11.4 | −0.150 |
| Bovine serum albumin | 69.00 | 4.8 | −0.433 |
| Carbonic anhydrase | 29.00 | 6.6 | −0.506 |
a
The set of proteins was selected based on their molecular weight, isoelectric point, and gravy index. The candidate proteins were also checked to not exhibit shared tryptic peptides with proteins from B. subtilis and the applied UPS2 standards. More detailed information is provided in Table S1.
b
pI, isoelectric point; MW, molecular weight.