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. 2024 Feb 16;12(4):e02339-23. doi: 10.1128/spectrum.02339-23

TABLE 3.

Primer sequences used for ARMS–LAMP analysisc,d,e

Primer Sequence (5′ → 3′) Length (bp)
armslampclr-F3 TCGGGTAAGTTCCGACCTG 19
armslampclr-B3 CGACTCCACACAAACTGGC 19
armslampclr-FIPa ACTCGTAGTGCAATTTCGCCGA-CGAATGGCGTAACGACTTCC 42
armslampclr-BIPaab AACGACCCCGGGACCTTCACT-GTGCTTCAAAGTCTCCCACC 41
armslampclr-BIPcab CACGACCCCGGGACCTTCACT-GTGCTTCAAAGTCTCCCACC 41
armslampclr-BIPgab GACGACCCCGGGACCTTCACT-GTGCTTCAAAGTCTCCCACC 41
armslampclr-BIPtab TACGACCCCGGGACCTTCACT-GTGCTTCAAAGTCTCCCACC 41
armslampclr-BIPacb ACCGACCCCGGGACCTTCACT-GTGCTTCAAAGTCTCCCACC 41
armslampclr-BIPagb AGCGACCCCGGGACCTTCACT-GTGCTTCAAAGTCTCCCACC 41
a

The FIP primer consists of F2 and the complementary strand of F1 (F1c).

b

The BIP primer consists of B2 and the complementary strand of B1 (B1c).

c

Underlined nucleotides show the location of each single-nucleotide polymorphism in 23S rRNA.

d

Nucleotides represented in bold letters show the locations of deliberate mismatches for ARMS.

e

bp, base pair; F3 and B3, outer primers; FIP and BIP, inner primers.