TABLE 3.
Primer | Sequence (5′ → 3′) | Length (bp) |
---|---|---|
armslampclr-F3 | TCGGGTAAGTTCCGACCTG | 19 |
armslampclr-B3 | CGACTCCACACAAACTGGC | 19 |
armslampclr-FIPa | ACTCGTAGTGCAATTTCGCCGA-CGAATGGCGTAACGACTTCC | 42 |
armslampclr-BIPaab | AACGACCCCGGGACCTTCACT-GTGCTTCAAAGTCTCCCACC | 41 |
armslampclr-BIPcab | CACGACCCCGGGACCTTCACT-GTGCTTCAAAGTCTCCCACC | 41 |
armslampclr-BIPgab | GACGACCCCGGGACCTTCACT-GTGCTTCAAAGTCTCCCACC | 41 |
armslampclr-BIPtab | TACGACCCCGGGACCTTCACT-GTGCTTCAAAGTCTCCCACC | 41 |
armslampclr-BIPacb | ACCGACCCCGGGACCTTCACT-GTGCTTCAAAGTCTCCCACC | 41 |
armslampclr-BIPagb | AGCGACCCCGGGACCTTCACT-GTGCTTCAAAGTCTCCCACC | 41 |
The FIP primer consists of F2 and the complementary strand of F1 (F1c).
The BIP primer consists of B2 and the complementary strand of B1 (B1c).
Underlined nucleotides show the location of each single-nucleotide polymorphism in 23S rRNA.
Nucleotides represented in bold letters show the locations of deliberate mismatches for ARMS.
bp, base pair; F3 and B3, outer primers; FIP and BIP, inner primers.