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. 2024 Mar 11;12(4):e03372-23. doi: 10.1128/spectrum.03372-23

TABLE 2.

The IC50, EC50, and CC50 values of cyclopropane-based compounds (C1c/d-C17c/d [40]) against FMDV 3Cpro in the enzyme and cell-based report assays

graphic file with name spectrum.03372-23.inline013.jpg
Compound R Z FRET assay
(IC50, µM)a 		Cell-based
assay (EC50 µM)b 		CC50 (µM)
C1c graphic file with name spectrum.03372-23.inline014.jpg -CHO 1.00 ± 0.28c 3.15 ± 0.35 >50
C1d -CH(OH)SO3Na 0.75 ± 0.07 3.25 ± 0.63 >50
C2c graphic file with name spectrum.03372-23.inline015.jpg -CHO 1.35 ± 0.64 1.20 ± 0.14 >50
C2d -CH(OH)SO3Na 1.10 ± 0.57 1.45 ± 0.21 >50
C3c graphic file with name spectrum.03372-23.inline016.jpg -CHO 0.69 ± 0.13 2.74 ± 2.14 >50
C3d -CH(OH)SO3Na 0.80 ± 0.14 4.02 ± 0.30 >50
C4c graphic file with name spectrum.03372-23.inline017.jpg -CHO 0.82 ± 0.15 2.74 ± 2.14 >50
C4d -CH(OH)SO3Na 1.35 ± 0.35 3.65 ± 0.63 >50
C5c graphic file with name spectrum.03372-23.inline018.jpg -CHO 1.05 ± 0.21 2.13 ± 0.49 >50
C5d -CH(OH)SO3Na 0.95 ± 0.22 1.89 ± 0.09 >50
C6c graphic file with name spectrum.03372-23.inline019.jpg -CHO 0.80 ± 0.05 3.30 ± 0.71 >50
C6d -CH(OH)SO3Na 0.58 ± 0.11 2.85 ± 0.49 >50
C7c graphic file with name spectrum.03372-23.inline020.jpg -CHO 0.59 ± 0.04 2.00 ± 0.14 >50
C7d -CH(OH)SO3Na 0.75 ± 0.07 2.15 ± 0.35 >50
C8c graphic file with name spectrum.03372-23.inline021.jpg -CHO 1.15 ± 0.08 NTd >50
C8d -CH(OH)SO3Na 0.55 ± 0.07 NT >50
C9c graphic file with name spectrum.03372-23.inline022.jpg -CHO 0.75 ± 0.14 2.95 ± 0.28 >50
C9d -CH(OH)SO3Na 0.84 ± 0.06 2.05 ± 0.64 >50
C10c graphic file with name spectrum.03372-23.inline023.jpg -CHO 1.25 ± 0.07 3.65 ± 1.48 >50
C10d -CH(OH)SO3Na 1.05 ± 0.21 2.85 ± 1.55 >50
C11c graphic file with name spectrum.03372-23.inline024.jpg -CHO 0.95 ± 0.21 1.08 ± 0.05 >50
C11d -CH(OH)SO3Na 0.45 ± 0.13 1.02 ± 0.17 >50
C12c graphic file with name spectrum.03372-23.inline025.jpg -CHO 0.50 ± 0.14 0.88 ± 0.25 >50
C12d -CH(OH)SO3Na 0.51 ± 0.06 1.25 ± 0.07 >50
C13c graphic file with name spectrum.03372-23.inline026.jpg -CHO 1.55 ± 0.07 2.95 ± 0.21 >50
C13d -CH(OH)SO3Na 0.75 ± 0.07 3.10 ± 0.42 >50
C14c graphic file with name spectrum.03372-23.inline027.jpg -CHO 0.63 ± 0.10 7.05 ± 0.78 >50
C14d -CH(OH)SO3Na 1.05 ± 0.64 6.30 ± 0.85 >50
C15c graphic file with name spectrum.03372-23.inline028.jpg -CHO 0.38 ± 0.11 0.45 ± 0.05 >50
C15d -CH(OH)SO3Na 0.29 ± 0.08 0.50 ± 0.11 >50
C16c graphic file with name spectrum.03372-23.inline029.jpg -CHO 0.36 ± 0.09 0.90 ± 0.06 >50
C16d -CH(OH)SO3Na 0.33 ± 0.04 1.21 ± 0.48 >50
C17c graphic file with name spectrum.03372-23.inline030.jpg -CHO 0.65 ± 0.07 >10 >50
C17d -CH(OH)SO3Na 0.75 ± 0.09 >10 >50
a

IC50, 50% inhibitory concentration.

b

EC50, 50% effective concentration; CC50, 50% cytotoxic concentration (values are M ± SD).

c

Mean (M) ± standard deviation (SD) of the means.

d

NT, not tested.