. 2024 Mar 7;12(4):e03885-23. doi: 10.1128/spectrum.03885-23

TABLE 3.

WC AGTC library preparation and sequencing cost comparison

Library preparation kit	Sequencing platform	Cost/sample (USD)^b
Nextera XT	MiSeq-v2-500 cycle	192.00
Nextera XT	NextSeq-Mid Output v2.5–300 cycle	112.00
Nextera Flex (1/4 vol)^a	MiSeq-v2-500 cycle	145.00
Nextera Flex (1/4 vol)^a	Miseq-v3-600 cycle	126.00
Nextera Flex (1/4 vol)^a	NextSeq-Mid Output v2.5–300 cycle	68.00

^{^a}

Nextera Flex quarter volume is an AGTC-developed protocol that uses a quarter of the full volume recommended by Illumina, plus additional modifications and optimizations.

^{^b}

Flat rate includes Illumina and non-Illumina reagents, consumables, TapeStation analysis, cost of library preparation for controls, samples that need to be repeated, and 15% administrative charge.