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The NS A(H1N1)pdm09 gene has been introduced multiple times in the swine population. Maximum clade credibility tree depicting TMRCA estimates of NS gene segment of human and swine IAVs. All Chilean swine NS sequences belong to the A(H1N1)pdm09-like lineage. Multiple introductions of the NS pandemic gene have been introduced into the swine population since the 2009 H1N1 pandemic.
