TABLE 6.
β-Lactamase genes and other potential resistance mechanisms identified in cefiderocol-resistant Acinetobacter spp.a isolates (n = 38)b,c
| Isolate | Country | FDC MIC (mg/L) | ST | Potential resistance mechanism(s) identified | |||||
|---|---|---|---|---|---|---|---|---|---|
| β-Lactamased | ftsI | carO | piuA | piuC | pirA-like | ||||
| 1 | Austria | 8 | 2 | ADC-74; OXA-66; OXA-72; NDM-1 | WT | No gross disruption | G216V; N489D; K658R | WT | WT |
| 2 | Austria | 4 | 2 | ADC-73; OXA-66; OXA-23 | A515V | No gross disruption | Gross disruption | WT | WT |
| 3 | France | 8 | 2 | ADC-85; OXA-66; OXA-23 | A515V | No gross disruption | Gross disruption | WT | WT |
| 4 | France | 8 | 2 | ADC-73; OXA-66; OXA-23 | A515V | No gross disruption | Gross disruption | WT | WT |
| 5 | France | 4 | 2 | ADC-73; OXA-66; OXA-23 | A515V | No gross disruption | Gross disruption | WT | WT |
| 6 | France | >32 | 1 | ADC-191; OXA-69; NDM-1; OXA-23 | L480I; T511S | No gross disruption | Gross disruption | WT | Gross disruption |
| 7 | France | 32 | 1 | ADC-191; OXA-69; NDM-1; OXA-23 | L480I; T511S | No gross disruption | Gross disruption | WT | Y479N; K543R |
| 8 | France | 16 | 85 | ADC-165; OXA-94; NDM-1 | WT | No gross disruption | Gross disruption (~56% ident to reference) |
WT | K475R; H566N |
| 9 | France | >32 | 2163e | ADC-2; OXA-132 | A512T | No gross disruption | I10F; N489D | WT | H566N |
| 10 | France | 8 | 1 | ADC-204; OXA-69; TEM-Trunc; OXA-23 | WT | No gross disruption | L76F; N489D | WT | Y479N; K543R |
| 11 | France | 32 | 85 | ADC-80; OXA-94; NDM-1 | WT | No gross disruption | Gross disruption (~56% ident to reference) |
WT | K475R; H566N |
| 12 | France | 4 | 203 | ADC-Trunc; OXA-65 | WT | No gross disruption | S10T; N19D; R42Q; L52I; N58T; A63S; T66Q; H67Q; Q90N; L119V; E125D; I164V; E185D; N206Q; S208E |
WT | T340I; H566N |
| 13 | Germany | >32 | 600 | ADC-73; OXA-66; TEM-1D; OXA-23 | A515V | No gross disruption | Gross disruption | WT | WT |
| 14 | Germany | >32 | 2 | ADC-30; OXA-66; PER-7; OXA-23 | WT | No gross disruption | G216V; N489D; K658R | WT | WT |
| 15 | Germany | 16 | 85 | ADC-176; OXA-94; NDM-1 |
WT | No gross disruption | Gross disruption (and ~56% ident to reference) |
WT | K475R; H566N |
| 16 | Germany | 4 | 636 | ADC-74; OXA-66; OXA-72 | WT | No gross disruption | Gross disruption | WT | WT |
| 17 | Germany | >32 | 2262e | ADC-11; OXA-66; OXA-72; PER-1 |
WT | No gross disruption | G216V; N489D; K658R | WT | WT |
| 18 | Italy | 16 | 2 | ADC-33; OXA-82; OXA-23 | H370Y | Gross disruption | G216V; N489D; K658R | WT | WT |
| 19 | Italy | 4 | 2 | ADC-73; OXA-66; OXA-23 | A515V | No gross disruption | G216V; N489D; K658R | WT | WT |
| 20 | Italy | 4 | 2 | ADC-33; OXA-82; OXA-23 | WT | No gross disruption | G216V; N489D; K658R | WT | WT |
| 21 | Italy | 16 | 2 | ADC-33; OXA-82; OXA-23 | WT | No gross disruption | G216V; N489D; K658R | WT | WT |
| 22 | Italy | 4 | 2 | ADC-33; OXA-82; OXA-23 | WT | No gross disruption | G216V; N489D; K658R | WT | WT |
| 23 | Italy | >32 | 2 | ADC-30; OXA-66; OXA-72 | WT | No gross disruption | G216V; N489D; K658R | WT | WT |
| 24 | Italy | 16 | 2 | ADC-30; OXA-66; OXA-72 | WT | No gross disruption | G216V; N489D; K658R | WT | WT |
| 25 | Italy | >32 | 2 | ADC-30; OXA-66; OXA-72 | WT | No gross disruption | G216V; N489D; K658R | WT | WT |
| 26 | Italy | >32 | 2 | ADC-30; OXA-66; OXA-72 | WT | No gross disruption | G216V; N489D; K658R | WT | WT |
| 27 | Italy | 16 | 600 | ADC-73; OXA-66; TEM-1D; NDM-1; OXA-23 |
A515V | No gross disruption | Gross disruption | WT | WT |
| 28 | Italy | 16 | 600 | ADC-73; OXA-66; TEM-1D; NDM-1; OXA-23 |
A515V | No gross disruption | Gross disruption | WT | WT |
| 29 | Italy | 16 | 600 | ADC-73; OXA-66; TEM-1D; NDM-1; OXA-23 |
A515V | No gross disruption | Gross disruption | WT | WT |
| 30 | Italy | 8 | 600 | ADC-73; OXA-66; TEM-1D; NDM-1; OXA-23 |
A515V | No gross disruption | Gross disruption | WT | WT |
| 31 | Italy | 8 | 600 | ADC-Trunc; OXA-66; TEM-1D; NDM-1; OXA-23 |
A515V | No gross disruption | Gross disruption | WT | WT |
| 32 | Italy | 16 | 600 | ADC-73; OXA-66; TEM-1D; NDM-1; OXA-23 |
A515V | No gross disruption | Gross disruption | WT | WT |
| 33 | Italy | 4 | 2 | ADC-33; OXA-82; OXA-23 | WT | No gross disruption | G216V; N489D; K658R | WT | WT |
| 34 | Italy | 4 | 2 | ADC-33; OXA-82; OXA-23 | WT | No gross disruption | G216V; N489D; K658R | WT | WT |
| 35 | Spain | 4 | 79 | ADC-1; OXA-65 | WT | No gross disruption | Gene not found | Gene not found | WT |
| 36 | Spain | >32 | 2 | ADC-30; OXA-66; OXA-23 | K235N | No gross disruption | G216V; N489D; K658R | WT | WT |
| 37f | UK | 8 | 432 | ADC-295; OXA-1189 | V72I; E115A; T179M; V343I; A345S; Q405E; A435V; E471Q; A483P; P604S; E605V | No gross disruption | Gross disruption | Gene not found | Gene not found |
| 38 | UK | >32 | 2 | ADC-73; OXA-66; TEM-1D; OXA-23 |
A515V | No gross disruption | G216V; N489D; K658R | WT | WT |
Isolates shown are A. baumannii unless otherwise indicated.
ADC, Acinetobacter-derived cephalosporinase; FDC, cefiderocol; MBL, metallo-β-lactamase; NDM, New Delhi MBL; OXA, oxacillinase; ST, sequence type; Trunc, truncated; UK, United Kingdom; WT, wild type.
Data were generated by whole-genome sequencing. A gene was considered to have a gross disruption if the coding sequence carried a nonsense mutation, frameshift, indels of >20 codons, or ablation of the canonical stop or start codons without a replacement immediately adjacent and in-frame. Genes were listed to be not found if a BLAST search with the reference gene yielded no hit with E-value <1E-25. Non-β-lactamase genes that were either found to have gross disruptions or mutations or were not found are shown in gray.
Data shown are a curated summary.
Indicates a novel ST.
A. calcoaceticus.