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. Author manuscript; available in PMC: 2024 Apr 2.

Published in final edited form as: Gastroenterology. 2023 Apr 5;165(1):201–217. doi: 10.1053/j.gastro.2023.03.228

Figure 2. — Spp1^{KI Mye} and WT mice were fed for 6 months with control or NASH-inducing diet. (A) H&E staining of liver (black arrows: macrovesicular steatosis; black open arrows: microvesicular steatosis; red arrows: inflammatory foci; blue arrows: hepatocyte ballooning degeneration). (B) Individual scores and NAS. (C) Oil red O staining of liver. (D) Liver TGs and CHO normalized by protein, and serum ALT activity. (E) Flow cytometry analysis of immune cells based on CD11b and F4/80 (left) and quantification of infiltrating MoMFs (n=3/genotype) (right). (F) IHC for COL1 (pink arrows: collagen fibrils). Results are expressed as mean ± SEM; n≥6/group. ^#p<0.05, ^##p<0.01 and ^###p<0.001 vs. control with same genotype; *p<0.05, **p<0.01 and ***p<0.001 vs. WT with same diet.

Figure 2. — Spp1^{KI Mye} and WT mice were fed for 6 months with control or NASH-inducing diet. (A) H&E staining of liver (black arrows: macrovesicular steatosis; black open arrows: microvesicular steatosis; red arrows: inflammatory foci; blue arrows: hepatocyte ballooning degeneration). (B) Individual scores and NAS. (C) Oil red O staining of liver. (D) Liver TGs and CHO normalized by protein, and serum ALT activity. (E) Flow cytometry analysis of immune cells based on CD11b and F4/80 (left) and quantification of infiltrating MoMFs (n=3/genotype) (right). (F) IHC for COL1 (pink arrows: collagen fibrils). Results are expressed as mean ± SEM; n≥6/group. ^#p<0.05, ^##p<0.01 and ^###p<0.001 vs. control with same genotype; *p<0.05, **p<0.01 and ***p<0.001 vs. WT with same diet.