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Published in final edited form as: Gastroenterology. 2023 Apr 5;165(1):201–217. doi: 10.1053/j.gastro.2023.03.228

Figure 6. — (A) Network landscape generated by Cytoscape indicating potential pathways leading to Arg2 upregulation (colors indicate subcellular localization; circle size indicates number of interactions). (B) Primary WT and Osmr null hepatocytes were cultured with conditioned medium of MFs from WT and Spp1^{KI Mye} mice. Western blot of pSTAT3, STAT3 and ACTB; n=4, ^##p<0.01 vs WT; **p<0.01 vs Ctrl siRNA. (C) ARG2 IF (yellow arrows: ARG2 expression in HEPs) and oil red O staining (red arrows: lipid droplets) of hepatocytes incubated with 30 μM BSA-conjugated-PA for 24 hours. (D) Spp1^{KI Mye} mice were injected an OSM neutralizing antibody or isotype control (0.25 μg/g, 10 doses every other day from the fourth week of NASH), while fed NASH-inducing diet. H&E staining of liver (black arrows: macrovesicular steatosis) (top) and co-localization of ARG2 and HNF4A (bottom, yellow arrows: ARG2⁺HNF4A⁺ cells). (E) Individual scores, NAS, liver TGs and CHO normalized by protein (n=3/group). *p<0.05 and **p<0.01 vs. Ctrl IgG.

Figure 6. — (A) Network landscape generated by Cytoscape indicating potential pathways leading to Arg2 upregulation (colors indicate subcellular localization; circle size indicates number of interactions). (B) Primary WT and Osmr null hepatocytes were cultured with conditioned medium of MFs from WT and Spp1^{KI Mye} mice. Western blot of pSTAT3, STAT3 and ACTB; n=4, ^##p<0.01 vs WT; **p<0.01 vs Ctrl siRNA. (C) ARG2 IF (yellow arrows: ARG2 expression in HEPs) and oil red O staining (red arrows: lipid droplets) of hepatocytes incubated with 30 μM BSA-conjugated-PA for 24 hours. (D) Spp1^{KI Mye} mice were injected an OSM neutralizing antibody or isotype control (0.25 μg/g, 10 doses every other day from the fourth week of NASH), while fed NASH-inducing diet. H&E staining of liver (black arrows: macrovesicular steatosis) (top) and co-localization of ARG2 and HNF4A (bottom, yellow arrows: ARG2⁺HNF4A⁺ cells). (E) Individual scores, NAS, liver TGs and CHO normalized by protein (n=3/group). *p<0.05 and **p<0.01 vs. Ctrl IgG.