Fig. 1.

Schematic representation of the tPA shRNA plasmid nanoparticle formulation administration to stroke-induced rats and the mechanism of tPA shRNA-mediated knockdown of tPA. Following the entry of plasmids into the nucleus of a cell, the CMV promoter and Drosha drive the formation of tPA pre-shRNA. Dicer processes tPA pre-shRNA, and the resulting tPA siRNA forms a complex with Ago and RISC. Ago, RISC, and tPA siRNA complex binds and destroys tPA mRNA and thereby prevents tPA protein translation. This figure was created with biorender.com under a paid subscription.