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Detection of JEV in BHK-21 cells. BHK-21 cells were inoculated with the supernatants harvested from the CPE-positive BHK-21 cells inoculated with mosquito samples and incubated for 36 h. The presence of JEV was detected by IFA (A) and Western blot (B) with antibodies specific to JEV NS3 protein. Nuclei were stained with DAPI.
