Fig. 3:

Podocyte Shp2 ablation mitigates hyperglycemia-induced renal ER stress, and inflammation. a, b Immunoblots of pPERK T981, PERK, peIF2α S51, eIF2α, pIRE1α S724, IRE1α, pNF-κB p65 S536, and NF-κB p65 (a), and mRNA expression of inflammatory cytokines IL1β, MCP1, and TNFα (b) in kidneys from Ptpn11^fl/fl and Ptpn11^fl/fl; Pod-Cre mice treated without (vehicle) and with STZ (160 μg/g body weight, 20 weeks). Each lane represents lysate from an individual animal. Phosphorylation levels of PERK, eIF2α, IRE1α, , and NF-κB p65 were normalized with their respective protein (n=6 each group), and mRNA expression was normalized to Tbp (n=6 in each group). *p ≤ 0.05, **p ≤ 0.01 vehicle versus STZ of mice with the same genotype. ^†p ≤ 0.05, ^††p ≤ 0.01 Ptpn11^fl/fl versus Ptpn11^fl/fl; Pod-Cre under the same treatment. A.U.: arbitrary unit.