Table 3. List of primers used for real-time quantitative PCR (rt-qPCR).
| Mouse primers: | ||
|---|---|---|
| Gene | Forward | Reverse |
| Slc16a1/Mct1 | TGTTAGTCGGAGCCTTCATTTC | CACTGGTCGTTGCACTGAATA |
| Slc16a1Mct1 (Exon 2,3 overlapping) | TGCAACGACCAGTGAAGTATC | GCTGCCGTATTTATTCACCAAG |
| Slc16a7/Mct2 | CCATCAGTAGTGTGTTGGTGAA | TCTATCACGCTGTTGCTGTAAG |
| Slc16a3/Mct4 | AGTGCCATTGGTCTCGTG | CATACTTGTAAACTTTGGTTGCATC |
| Srebf1 | GGAGCCATGGATTGCACATT | GGCCCGGGAAGTCACTGT |
| Mlxipl | TCTGCAGATCGCGTGGAG | CTTGTCCCGGCATAGCAAC |
| Fasn | GGAGGTGGTGATAGCCGGTAT | TGGGTAATCCATAGAGCCCAG |
| Scd1 | CCGGAGACCCCTTAGATCGA | TAGCCTGTAAAAGATTTCTGCAAACC |
| Acly | TGGTGGAATGCTGGACAA | GCCCTCATAGACACCATCTG |
| Tgfb1 | CTCCCGTGGCTTCTAGTGC | GCCTTAGTTTGGACAGGATCTG |
| Ihh | CTCTTGCCTACAAGCAGTTCA | CCGTGTTCTCCTCGTCCTT |
| Acta2 | ATGCTCCCAGGGCTGTTTTCC | GTGGTGCCAGATCTTTTCCATGTCG |
| Gli2 | CAACGCCTACTCTCCCAGAC | GAGCCTTGATGTACTGTACCAC |
| Gli3 | CACAGCTCTACGGCGACTG | CTGCATAGTGATTGCGTTTCTTC |
| Col1a1 | GCTCCTCTTAGGGGCCACT | CCACGTCTCACCATTGGGG |
| Col1a2 | GTAACTTCGTGCCTAGCAACA | CCTTTGTCAGAATACTGAGCAGC |
| Col3a1 | CTGTAACATGGAAACTGGGGAAA | CCATAGCTGAACTGAAAACCACC |
| Timp1 | CTCAAAGACCTATAGTGCTGGC | CAAAGTGACGGCTCTGGTAG |
| Alb | TGCTTTTTCCAGGGGTGTGTT | TTACTTCCTGCACTAATTTGGCA |
| Des | CTAAAGGATGAGATGGCCCG | GAAGGTCTGGATAGGAAGGTTG |
| Clec4f | GAGGCCGAGCTGAACAGAG | TGTGAAGCCACCACAAAAAGAG |
| B2m | CATGGCTCGCTCGGTGAC | CAGTTCAGTATGTTCGGCTTCC |
| F4/80 | CTTTGGCTATGGGCTTCCAGTC | GCAAGGAGGACAGAGTTTATCGTG |
| Ccl2 | AGGTCCCTGTCATGCTTCTG | AAGGCATCACAGTCCGAGTC |
| Il1b | TTTGACAGTGATGAGAATGACC | CTCTTGTTGATGTGCTGCTG |
| Tlr4 | ATGGCATGGCTTACACCACC | GAGGCCAATTTTGTCTCCACA |
| Ccr2 | ATCCACGGCATACTATCAACATC | CAAGGCTCACCATCATCGTAG |
| Ccl20 | GCCTCTCGTACATACAGACGC | CCAGTTCTGCTTTGGATCAGC |
| Cd163 | ATGGGTGGACACAGAATGGTT | CAGGAGCGTTAGTGACAGCAG |
| Arg1 | CTCCAAGCCAAAGTCCTTAGAG | AGGAGCTGTCATTAGGGACATC |
| Ccl22 | AGGTCCCTATGGTGCCAATGT | CGGCAGGATTTTGAGGTCCA |
| Cd206 | CTCTGTTCAGCTATTGGACGC | TGGCACTCCCAAACATAATTTGA |
| Human primers: | ||
| Gene | Forward | Reverse |
| SLC16A1/MCT1 | TGGAAGACACCCTAAACAAGAG | AAAGCCTCTGTGGGTGAATAG |
| ACTA2 | AGCGTGGCTATTCCTTCGT | CTCATTTTCAAAGTCCAGAGCTACA |
| TGFB1 | CAACGAAATCTATGACAAGTTCAAGCAG | CTTCTCGGAGCTCTGATGTG |
| COL1A1 | ACGTCCTGGTGAAGTTGGTC | ACCAGGGAAGCCTCTCTCTC |
| TIMP1 | AATTCCGACCTCGTCATCAGG | ATCCCCTAAGGCTTGGAACC |
| ACTB | GATGAGATTGGCATGGCTTT | GAGAAGTGGGGTGGCTT |