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. 2024 Apr 3;14(4):240007. doi: 10.1098/rsob.240007

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© 2024 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 4. — Construction of the Mirc56 random mutant mES clone library. (a) Workflow for library construction. (b) Random integrated sgRNA-Mirc56_X cassettes in bulk PB mES cells. The bar colour shows each sgRNA-Mirc56_X cassette. The y-axis represents the percentage of reads with targeted amplicon short next-generation sequencing (NGS). (c) EGFP-based single-cell sorting in bulk PB mES cells. The upper table shows the conditions of transfection. Lower scatter graphs show each mES cell passing quality filters in a total of 2000 events. The y-axis shows the EGFP signal intensity. The boxes in scatter graphs show the gates of the EGFP filter.