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. Author manuscript; available in PMC: 2024 Oct 2.
Published in final edited form as: Mol Cancer Res. 2024 Apr 2;22(4):329–336. doi: 10.1158/1541-7786.MCR-23-0749

Figure 1. AA PDX models are comprised of human tumor and mouse stroma.

Figure 1.

(A) Computationally merged 40x magnification immunohistochemistry (IHC) staining of peritoneal and flank-implanted tumors using human marker KU80 (red), GI epithelial marker CDX2 (gold), and stromal marker Vimentin (green). Overlap of KU80 and CDX2 is orange. Representative H&E images are also shown. (B) ESTIMATE Stromal Score for each sample’s transcriptome split by assignment to either human reads or mouse reads, black bars represent mean (Wilcoxon p<0.0001). (C) Volcano plot displaying differentially expressed genes (DEGs) from peritoneal tumor microenvironment (TME) vs. flank TME. Points were colored based on overlap with differentially expressed genes (DEGs) from normal human visceral omentum vs. human subcutaneous adipose tissues. PDX unique DEGs are genes that were DEGs in the PDX TME comparison but were not differentially expressed in the human tissue comparison (D) PCA plots made using the top 500 genes by variance displaying clustering of human tumor reads (top) and mouse TME reads (bottom).