Figure 3. Peritoneal microenvironment promotes Hippo pathway inhibition but does not affect tumor-intrinsic expression of Mucin Genes.

(A) Volcano Plot from differential protein expression analysis comparing implant site using reverse phase protein array (RPPA) data. Inset shows the ratio of active, unphosphorylated YAP to inactive, phosphorylated YAP for each of the samples, as measured by normalized and linearized EC50 values. (B) Average normalized counts from RNA-Seq for all mucin genes expressed (total normalized counts ≥ 100), split by tumor implanted and implant site. The color shows the z-transformed average reads for each gene while the size shows the average normalized counts without z-transformation. (C) Chromogenic Immunohistochemistry for MUC1 (brown) split by implanted model (columns) and implant site (rows).