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. Author manuscript; available in PMC: 2024 Oct 2.
Published in final edited form as: Mol Cancer Ther. 2024 Apr 2;23(4):478–491. doi: 10.1158/1535-7163.MCT-23-0312

Fig.2: QC6352 causes cell cycle arrest and DNA damage in WiT49 and HEK293 cells.

Fig.2:

(A) Propidium iodide staining with flow cytometry demonstrates an increased proportion of QC6352-treated (25nM for 72 hours) cells in the S-phase (paired two-tailed t-test p=0.0097 WiT49; p=0.0175 HEK293) and reduced proportion in the G0G1 phase (p=0.0274 WiT49; p=0.0079 HEK293). (B) QC6352 (100nM for 72 hours) induces DNA damage detected by the Comet assay. White arrows show “comet tails” indicative of DNA damage. Images were taken at 20X magnification. Doxorubicin-treated cells (10nM for 72 hours) are included as positive control. Kruskal-Wallis p-values are represented. (C) Western blot analysis for DNA damage and activation of checkpoint response proteins. Cells were treated with QC6352 (25nM) for 72 hours. Doxorubicin-treated cells (10nM for 72 hours) are included as a positive control. GAPDH is used as a loading control.