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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: J Autoimmun. 2023 Oct 4;140:103119. doi: 10.1016/j.jaut.2023.103119

Figure 4. SOCS1 mutants and interaction with JAK1.

Figure 4.

A) Immunoblot and densitometry analysis of JAK1/SOCS1 in transfected HEK293T cells before or after immunoprecipitation using anti-FLAG beads. The ratio of JAK1/SOCS1 expression is displayed. B) Immunoblotting and immunoprecipitation for the analysis of JAK1/SOCS1 interaction in IFN-α2-treated cells. Bands were quantified by densitometry analysis using Image J Software. Bar graphs illustrate the median of 6 independent experiments in panel A and 5 independent experiments in panel B. p < 0.05 (Mann Whitney U test).