Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 8;20(4):374–402. doi: 10.1038/s44320-024-00024-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

Figure EV5 — (A) Bar plot displays mean of basal, minimum, and maximum oxygen consumption rate in HepG2 cells with control (siNT, dark gray) and siRNA-mediated TEAD1-KD (siTEAD1, light gray) (n = 63 per condition, +SD). Dots indicate single wells across two biological replicates. Significant P values are indicated (two-sided t test). (B) Bar plot shows the adjusted P values (log₁₀) of the top 12 KEGG pathways enriched for genes deregulated upon TEADap treatment. Red vertical line indicates adjusted P value threshold (P < 0.05). Color gradient indicates gene number per pathway (low: gray, high: black). One-sided Fisher’s exact test with Benjamini–Hochberg correction was used for the overrepresentation analysis. (C) Heatmap displays gene expression changes of the top 12 KEGG pathways altered upon TEADap treatment. Left panel indicates normalized gene expression (z-score) in free fatty acid-fed controls, TEADsf and TEADap treatments (low: blue, high: red). Right panel shows log₂FC for ER-agonist treatments compared to untreated male mice on HFD (low: green, high: brown). Gene names follow human nomenclature. Ortholog absence in mouse is specified (gray). (D) Genome browser views (IGV, hg38) show genomic regions around SREBF1, HMGCR and GHR gene loci. Genomic locations and sizes are indicated. The y axis of each track specifies normalized ChIP-seq read density (parenthesis) of YAP (pink), TAZ (purple) or TEAD1 (blue) in HUVEC cells (GSE163458). Vertical lines indicate TEAD1-binding sites identified by motif search. Blue boxes represent exons and UTRs, connecting lines indicate intronic sequences. Arrows indicate directionality of gene transcription. (E) Density plot shows the distribution of overlaps between 1000 random sets of genes unchanged by TEADap treatment and genes changed by ER-agonist treatments falling within the top 12-enriched KEGG pathways (gray peak). The percentage of overlapping genes changed in both TEADap and ER-agonist treatments for the top 12-enriched KEGG pathways is displayed (black vertical line). P value is indicated (permutation tests).