Table 4.

Box-Behnken design portfolio and results.

Serial number	Substrate concentration (C)	Inoculum volume (mL)	Temperature (°C)	CMCase activity (U/mL)
1	1	2	37	4.63
2	3	2	37	6.53
3	1	8	37	5.03
4	3	8	37	4.69
5	1	5	30	4.66
6	3	5	30	12.04
7	1	5	44	3.14
8	3	5	44	6.79
9	2	2	30	5.22
10	2	8	30	3.89
11	2	2	44	2.66
12	2	8	44	3.82
13	2	5	37	14.71
14	2	5	37	17.63
15	2	5	37	16.17
16	2	5	37	15.42
17	2	5	37	16.92

CMCase activity calculated based on experimental results.