Table 5.

Box-Behnken test results of isolates.

Source	sum of squares	Df	Mean square	F value	P value Prob > F	Significant
Model	472.94	9	52.55	19.42	0.0004	Significant
A-substrate concentration	19.81	1	19.81	7.32	0.0304	*
B-inoculum volume	0.32	1	0.32	0.12	0.7395
C-temperature	11.05	1	11.05	4.08	0.0831
AB	1.25	1	1.25	0.46	0.5178
AC	3.48	1	3.48	1.29	0.2942
BC	1.55	1	1.55	0.57	0.4738
A2	70.61	1	70.61	26.09	0.0014	**
B2	197.86	1	197.86	73.12	< 0.0001	**
C2	123.58	1	123.58	45.67	0.0003	**
Residual	18.94	7	2.71
Lack of fit	13.55	3	4.52	3.35	0.1365	Not significant
Pure error	5.39	4	1.35
Cor total	491.88	16

*P < 0.05, significant difference; **P < 0.01, extremely significant difference.