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. 2024 Feb 23;43(7):1164–1186. doi: 10.1038/s44318-024-00055-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV2 — (A) LDH absorbance in WT and Ninj1^−/− BMDMs untreated (UT) or lysed with Triton X-100 to a final concentration of 0.01% (max. lysis). (B) Western blot of NINJ1 expression in WT or Ninj1^−/− BMDMs transduced with a retroviral vector expressing WT mNINJ1 or different mNINJ1 mutants. Transduction with a GFP expressing vector was used as a control. Cell extracts were analyzed. GAPDH is a loading control. (C, D) Immunofluorescence confocal microscopy of NINJ1 (red) in WT or Ninj1^−/− BMDMs complemented with WT or different NINJ1 mutants upon retroviral transduction. Scale bars: 20 µm (C) and 60 µm (D). (E) LDH release in WT and Ninj1^−/− BMDMs upon treatment with 1 mM CuOOH for 8 or 14 h. Data information: All graphs show the mean ± SD. Data are pooled from three independent experiments performed in triplicate (A, E) or representative of two different experiments (B, C, D). Statistical analysis was done using Student’s unpaired two-sided t-test. **** P < 0.0001, ** < 0.01.