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. 2024 Apr 2;15:2837. doi: 10.1038/s41467-024-47092-z

Fig. 3. Scratch1 expression is restricted to the neural lineage.

Fig. 3

a In situ hybridisation for Scratch1 (green) combined with immunohistochemistry for nucleoporins (red) in cultured NSCs (left), ESCs (center) and iPSCs (right). b Relative mRNA levels of Scratch1 in RNA extracts obtained from NSCs, ESCs and iPSCs (n = 3 biologically independent samples). c Schematic representation of the protocol to obtain ESC-derived NSCs. d Cells at different time points of the ESC-to-NSC differentiation process observed under phase contrast optics. GFP (green) labels the cells that express Sox1. Relative mRNA levels of several pluripotency markers (Nanog, Pou5f1 and Zfp42, e); neural markers (Sox1 and Nestin, f); and Scratch1 (g) in RNA extracts obtained at different time points during the differentiation of ESCs into NSCs (n = 4 independent experiments). Scale bars: (a),10 µm; and (d), 100 µm. DIV, days in vitro. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.