Table 2.

Pros and cons of different routes of administration of rAAV

Routes of administration	Pros	Cons	Examples
Intravascular	• Widespread transduction	• Transduction of non-target organs	i.v., i.a.
	• Reach most organs	• Potentially high doses required
Intra-tissue	• Localized delivery	• Invasive	i.m., i.p.
		• Decreased spread
		• Possible local reaction
Intra-cavity	• Spread in preformed space	• Transduction potentially limited to preformed space	s.p., s.r., i.v.t
		• Potentially uneven distribution
Intra-fluid space	• Utilization of fluid dynamics to distribute vector	• Long vector travel distance	i.c.v., c.m., i.t.

i.v. intravenous, i.a. intra-arterial, i.c.v. intracerebroventricular, i.m. intramuscular, i.t. intrathecal delivery, c.m. cisterna magna, i.p. intraparenchymal, s.p. subpial space, s.r. subretinal, i.v.t. intravitreal