Table 1.
Antibodies used for immunophenotyping of the cells in the peritoneal cavity.
| Target | Fluorophore | Clone | Vendor | Dilution | FMO |
|---|---|---|---|---|---|
| Common | |||||
| CD16/CD32 | – | 93 | eBioscience | 1:100 | – |
| CD45 | APC-eFluor 780 | 30-F11 | eBioscience | 1:100 | |
| Lymphoid panel | |||||
| CD3 | Alexa Fluor 700 | 17A2 | BioLegend | 1:100 | – |
| CD4 | PerCP-Cy5.5 | GK1.5 | BioLegend | 1:150 | – |
| CD8a | PE-Cy7 | 53-6.7 | BioLegend | 1:150 | – |
| CD19 | Brilliant Violet 421 | 6D5 | BioLegend | 1:100 | – |
| PD-1 | PE | 29F.1A12 | BioLegend | 1:80 | yes |
| NK1.1 | FITC | PK136 | BioLegend | 1:80 | yes |
| Myeloid panel | |||||
| CD11b | PE-Cy7 | M1/70 | BioLegend | 1:120 | yes |
| CD64 | FITC | X54-5/7.1 | BioLegend | 1:80 | yes |
| Ly6G | APC | 1A8 | BioLegend | 1:100 | – |
| Ly6C | PE-Dazzle 594 | HK1.4 | BioLegend | 1:100 | yes |
| SiglecF | PerCP-Cy5.5 | S17007L | BioLegend | 1:80 | – |
| PD-L1 | PE | 10F.9G2 | BioLegend | 1:80 | yes |