Table 1.
Purification of rVAMT.
| Step | Total activity (U) | Total protein (mg) | Specific activity (U/mg)* | Yield (%) |
Purification (-fold) |
|---|---|---|---|---|---|
| Crude | 78.1 | 1020 | 0.0765 | 100 | 1 |
| TALON | 37.9 | 33.2 | 1.14 | 48.5 | 14.9 |
| thrombin | 47.1 | 33.2 | 1.42 | 60.3 | 18.5 |
| Butyl-Toyopearl | 7.59 | 1.73 | 4.38 | 9.71 | 57.3 |
V-forming activity from VA using pyruvate as an amino acceptor.