Table 2.

Kinetic parameters of rVAMT.

Varying	Fixed	Km	Vmax	kcat	kcat/Km
substrate	substrate	(mM)	(U/mg protein)	(s−1)	(s−1/mM)
VA	Na pyruvatea	0.25 ± 0.037	2.9 ± 0.11	2.5	9.8
V	GABAa	0.48 ± 0.049	1.7 ± 0.051	1.4	3.0
V	L-Alaa	0.12 ± 0.019	0.91 ± 0.040	0.77	6.4
Na pyruvate	VAb	5.4 ± 0.34	2.7 ± 0.052	2.3	0.43
GABA	Vb	36 ± 2.1	1.5 ± 0.023	1.3	0.036
L-Ala	Vb	160 ± 7.0	1.1 ± 0.020	0.93	0.0058
VAG	Na pyruvatea	15 ± 4.8	2.3 ± 0.38	1.9	0.13
VG	GABAa	3.3 ± 0.56	0.42 ± 0.029	0.36	0.11
VG	L-Alaa	1.5 ± 0.37	0.38 ± 0.029	0.32	0.21

Data the mean ± SEM (SEM: fitting error to the Michaelis-Menten equation as calculated the non-linear fitting program).

^aAt 100 mM concentration.

^bAt 5 mM concentration.