FIG. 5.

Effect of Act D on RNase H activity in the absence of NC. In vitro strand transfer reaction mixtures were incubated with donor RNA labeled at its 5′ end with ³²P and increasing concentrations of Act D, as described in Materials and Methods. (A) Gel analysis. (B) Quantitative PhosphorImager analysis. The amount of the 8-nt RNA cleavage product was plotted against the concentration of Act D. Lanes: 2, no drug; lanes 3 to 8, Act D at 1, 5, 10, 20, 40, and 80 μM, respectively. Lane 1 is a control showing the position of the uncleaved labeled donor RNA from a reaction without RT and Act D.