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. 2024 Mar 11;15(9):2561–2572. doi: 10.7150/jca.92386

Figure 2.

Figure 2

USP15 interacts with HMGB1. A: Coomassie brilliant blue stained protein co-precipitated with HMGB1. B: Mass spectrometry analysis of HMGB1-binding protein. In this study, we have listed ten potential HMGB1-interacting proteins based on the Protein peptide abundance. HMGB1, HNRNPU, and USP15 are the most enriched protein in this study. C: Co-IP (Co-immunoprecipitation) assay of USP 15 and HMGB1 in HEK-293T cells transfected with USP15 and HMGB1 overexpression. D: Co-IP assay of USP 15 and HMGB1 in B-CPAP cells and KTC-1 cells. E: USP15 and HMGB1 expression and subcellular localization were detected using immunofluorescence in B-CPAP cells and KTC-1 cells.