FIGURE 1.

Schematic overview of the study. (a) Test day protocol commenced with a pre‐exercise breakfast ∼2 h prior. One hour before exercise, subjects inserted a telemetric pill rectally before a pre‐exercise blood sample was collected. Subjects completed a 15‐min warm‐up before the commencement of the exercise test. Upon completion of the test, a post‐exercise sample was collected, and all samples were processed and stored immediately. (b) Sample preparation and ¹H‐NMR analysis with peaks assigned using Chenomx; the entire parameter set raw and processed spectra are deposited in the EMBL‐EBI repository MetaboLights. (c) Univariate and multivariate analyses of metabolomics data were performed in R, and pathway analysis was completed using MetaboAnalyst. Created with BioRender.com.