Fig. 2. (A) Reaction pathway of cleavage and photobleaching of 1 followed by 3D fluoresce spectroscopy in THF. The cleavage reaction was performed in water for 25 min with λmax = 415 nm, resulting in an increasing intensity of the bimane emission (B) compared to (C). To photo-bleach NBD, 1 was irradiated in water for 13 h with λmax = 505 nm, resulting in an increasing intensity of the bimane emission (D).
