Fig. 3. (A) Post-functionalization of P1 with 5 and 2 to incorporating the FRET pair. 2 concomitantly acts as cross-linker, folding P2 into SCNP1. (B) RID SEC traces of P1 post-functionalized to P2 and further folding to SCNP1. (C) UV/Vis absorption SEC trace at 380 nm and 450 nm normalized by RID of P1, P2 and SCNP1. (D) Kinetics of the post-functionalization of P1 to P2 and folding of P2 to SCNP1 monitored by UV/Vis absorption and Mn by SEC. The cross-linking of the polymer chain leads to a compaction and consequently to a smaller hydrodynamic volume thus to a smaller apparent molecular weight in the SEC. Mn: apparent number average molecular weight.
