Fig. 5. (A) Illustration of SCNP1 irradiated with λmax = 505 nm for 8 h resulting in photobleaching of the NBD and SCNP2 irradiated with λmax = 415 nm for 3 h resulting in the cleavage and bleaching of the bimane units. (B) SCNP1 and SCNP2 analysed by SEC showing different absorption at 450 nm and 380 nm. (C) The apparent Mn (red) increases after irradiating with λmax = 415 nm in water indicating unfolding of the SCNPs, whereas the apparent Mn does not increases after irradiating with λmax = 505 nm. (D) 3D fluorescence spectrum of SCNP1 irradiated with 505 nm for 8 h in water. (E) RID SEC traces of SCNP2 unfolding compared to P1. Mn: apparent number average molecular weight.
