FIG. 6.

Effect of MT5/6 on Pol expression. (A) In the terminal redundant R9 construct, an RT-Pol-LacZ fusion gene was generated by exchanging a major part of the RT-Pol ORF with a cDNA fragment of lacZ. (B) After transfection of the adw or MT5/6 RT-Pol fusion construct into HuH-7 cells, expression of the RT-Pol-LacZ fusion protein was analyzed by SDS-PAGE (10% gel) and immunoblotting with an anti-LacZ antibody (top). Analysis of LacZ expression of plasmid pCDLacZ served as positive control. The lower band of the pCDLacZ lane probably represents a degradation product of the full-length LacZ protein. The blot was stripped and reprobed with an antiactin antibody (bottom). Positions of molecular weight (MW) markers (in kilodaltons) are indicated on the left. Transfection efficiencies as monitored by pTKGH cotransfection were similar among all samples.