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. 2024 Apr 3;43:103. doi: 10.1186/s13046-024-03015-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — MSLN promotes the migration and invasion of NSCLC cells in vitro. A Representative images and quantitative results of western blotting showing MSLN expression after transfection of PC9-BrM and PC9 cells with lentivirus. B The effect of MSLN expression on the migration capacity in PC9-BrM and PC9 cells assessed in a wound-healing assay (scale bar, 100 µm). C Transwell migration and invasion assays to determine the effect of altered MSLN expression on the migration and invasion of NSCLC cells (scale bar, 200 µm). D, E Western blot analysis of E-cadherin, N-cadherin, Slug and MMP7 expression in PC9-BrM cells and PC9 cells after alteration of MSLN expression. F-G The expression of MMP7 by PC9 cells and PC9-BrM cells was detected by qRT-PCR (F) and ELISA (G). (PC9-NC, PC9 cells transfected with negative control plasmid. PC9-OE, PC9 cells transfected with MSLN plasmid. PC9-BrM-NC, PC9-BrM cells transfected with negative control shRNA. PC9-BrM-SH1, PC9-BrM cells transfected with MSLN-targeted shRNA1. PC9-BrM-SH2, PC9-BrM cells transfected with MSLN-targeted shRNA2. Data are presented as mean ± SD, ns, no significance)