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. 2024 Apr 3;43:103. doi: 10.1186/s13046-024-03015-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — MSLN promotes NSCLC cells penetration of endothelium by promoting cleavage of endothelial TJ proteins. A Schematic diagrams of a classic in vitro blood-brain barrier (BBB) model and the multi-organ microfluidic chip. B, C Representative images of the ability of tumor cells to penetrate the BBB in the Transwell assay and the chip (scale bar, 200 µm). D, E Representative confocal microscopy images showing the distribution of VE-cadherin, JAM-A and claudin-5 in a hBMVEC monolayer (scale bar, 20 µm). F, G Western blot analysis of VE-cadherin, JAM-A and claudin-5 expression in hBMVECs after treatment with conditioned medium from the indicated tumor cells. (TJ, tight junction. PC9-NC, PC9 cells transfected with negative control plasmid. PC9-OE, PC9 cells transfected with MSLN plasmid. PC9-BrM-NC, PC9-BrM cells transfected with negative control shRNA. PC9-BrM-SH1, PC9-BrM cells transfected with MSLN-targeted shRNA1. PC9-BrM-SH2, PC9-BrM cells transfected with MSLN-targeted shRNA2. Data are presented as mean ± SD, ns, no significance)