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. 2024 Apr 3;43:103. doi: 10.1186/s13046-024-03015-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The effect of MSLN on NSCLC penetration of the BBB is dependent on MET. A,B Representative images and quantification analysis of MSLN and MET staining in primary lung tumor (LT, n=25) and lung cancer-derived brain metastases (BM, n=25) surgical specimens (scale bar, 200 μm). C Correlation of MSLN and MET protein expression in BM surgical specimens. D, E Representative western blot images showing the expression of MSLN, p-MET and MET in the indicated cells. F, G Representative images and quantitative results of western blotting for MET expression in tumor cells after transfection. H Effect of MET on the ability of NSCLC cells to penetrate the endothelium. I, J After treatment of hBMVECs with conditioned medium from the designated tumor cells for 24 h, western blot analysis and immunofluorescence detection showed the distribution of VE-cadherin, JAM-A and claudin-5 expression in hBMVEC monolayers (scale bar, 20 μm). (PC9-NC, PC9 cells transfected with negative control plasmid. PC9-OE, PC9 cells transfected with MSLN plasmid. PC9-BrM-NC, PC9-BrM cells transfected with negative control shRNA. PC9-BrM-SH1, PC9-BrM cells transfected with MSLN-targeted shRNA1. PC9-BrM-SH2, PC9-BrM cells transfected with MSLN-targeted shRNA2. PC9-BrM-SIR-1, PC9-BrM cells transfected with MET-targeted siRNA-1. PC9-BrM-SIR-2, PC9-BrM cells transfected with MET-targeted siRNA-2. PC9-BrM-SH2-NC, PC9-BrM-SH2 cells transfected with negative control plasmid. PC9-BrM-SH2-OE, PC9-BrM-SH2 cells transfected with MET plasmid. MET-SIR-1, PC9-BrM cells transfected with MET-targeted siRNA-1. shMSLN, PC9-BrM cells transfected with MSLN-targeted shRNA1. MET-OE, PC9-BrM cells transfected with MET plasmid.Data are presented as mean ± SD, ns, no significance)