Table 2.

Primer pairs used for RT-PCR and qPCR

Gene name	Accession number	Orientation	Nucleotide sequence	Nucleotide position	Product Length (bp)	Annealing Temp (℃)
Map1a	NM_030995.2	Sense Anti-Sense	5’-AAACGGAGGCTGATCAAGGA-3’ 5’-TTCAGGTCTGGCTTGGAGAG-3’	1174–1193 1375–1394	221	60.0
Map1b	NM_019217.1	Sense Anti-Sense	5’-GGAAGAAGACGGGGAAGACA-3’ 5’-GTCCTCTGCTTTGTCCTCCT-3’	2841–2860 3047–3066	226	60.0
Map1s	NM_001106070.1	Sense Anti-Sense	5’-CCCTGGAGAAGCAGAGAACA-3’ 5’-GACTCGTCCACCTCAGTTGA-3’	1653–1672 1873–1892	240	60.0
Map2	NM_013066.1	Sense Anti-Sense	5’-TCTCAGTTCAGTGCCAGAGG-3’ 5’-TTTCCTGAGGTGCTTCGGAT-3’	3051–3070 3252–3271	221	60.0
Map4	NM_039082634.1	Sense Anti-Sense	5’-GGGTGTGTTTTTGAGCAGGT-3’ 5’-GCAGGCTCCAAGCATAGTTC-3’	8387–8406 8566–8585	199	60.0
Map6	NM_017204.1	Sense Anti-Sense	5’-CAGCTACAGTGCCCAGTTCA-3’ 5’-TCTGGCTCGTTGAGGTCTTT-3’	2026–2045 2179–2198	173	60.0
Map7	NM_001106270.2	Sense Anti-Sense	5’-GAATGGAATTTCAGGCTCCA-3’ 5’-ATGCCACCTCAGGGTATCAG-3’	2468–2487 2629–2648	181	60.0
Map9	NM_001135716.1	Sense Anti-Sense	5’-TGATCAAGACGTTGGCAGAG-3’ 5’-GGGCAGAATCTTCGGTTGTA-3’	608–627 812–831	224	60.0
Map10	NM_001277391.1	Sense Anti-Sense	5’-ATTTTGCCCTCCTCCTCTGT-3’ 5’-CCCTGCATCTGTGGAAGATT-3’	805–824 1007–1026	222	60.0
Map6d1	NM_001108844.1	Sense Anti-Sense	5’-GAAGCCCTCAAGATCCACAA-3’ 5’-GAGGGGCTGATGTCTGAAAG-3’	416–435 548–567	152	60.0
Map7d3	NM_001271327.1	Sense Anti-Sense	5’-TCCTGTTGTCAGCTTGGATG-3’ 5’-GGTGCTTGTTTCTTGGTGGT-3’	1107–1126 1336–1355	249	60.0
Kif15	NM_181635.2	Sense Anti-Sense	5’-CAGCTTCAGCTGGACAATGC-3’ 5’-TGTTGCGCTCTTTTTCTGCC-3’	2547–2566 2741–2760	214	60.0
S16	NM_001169146.1	Sense Anti-Sense	5’-TCCGCTGCAGTCCGTTCAAGTCTT-3’ 5’-GCCAAACTTCTTGGATTCGCAGCG-3’	87–110 448–471	385	60.0