TABLE 1.

Summary of transformation assays of Myb proteins in the presence/absence of E9A

Protein	Transformation ability		Expression of cell surface marker in transformed hematopoietic cellsa
	Liquid culture outgrowth (no. of cells/ml on day 21)b	Methocel assay (no. of colonies)c	HLO72	1C3
N-Cla	<104	3	ND	ND
dVd	6.3 × 106	117	+	−
dVdE	5.1 × 106	109	+	−
dCd	7.7 × 106	128	+	+
dCdE	5.6 × 106	119	+	+
dCC	3.7 × 106	71	+	+
dCCE	3.1 × 106	59	+	+
CCC	2.1 × 105	27	+	+
CCCE	1.8 × 105	26	+	+
CCd	1.6 × 105	23	+	+
CCdE	1.3 × 105	20	+	+

^aCell surface markers HLO72 (a monoblast-specific marker [38]) and 1C3 (a granulocyte-specific marker [40]) were assayed by FACS analyses using the relevant antibodies. ND, not determined; −, not detectable; + detectable (≥85% of the cells stained positively for the cell surface marker). 

^bTransformed cells were counted on day 21. Data from one representative experiment are shown. 

^c10⁵ cells from each culture were seeded into 0.8% methocel on day 5 of the transformation assays, and colonies were counted after 2 weeks. Data from one representative experiment are shown.