TABLE 1.
Apoptotic fraction in total population of CHO4.15 cells transfected with p53 and Bcl2 constructs (as measured by TUNEL assay)
Cells | Apoptotic fraction (%)
|
|
---|---|---|
No Bcl2 cotransfected | 250 ng of Bcl2 cotransfected | |
Control (no p53 transfected) | NDa | 2.9 |
Transfected with p53 construct: | ||
wt p53 | 19.2 | 6.0 |
ΔN39ΔC362 | 2.3 | 6.7 |
ΔN39ΔC362Trp248 | 4.8 | ND |
ND, not determined.