TABLE 1.

Primers used in site-directed mutagenesis

No.	Sequence	Resulting mutanta
1	5′-GTCTAAACATACACGGTACCTTTC-3′
2	5′-CCAAACATTATGAATAGTAC-3′	S2N
3	5′-AAGGTACCAAACATTATGGCTGCTAC	A2A3
4	5′-AAGGTACCAAACATTATGAGTAGTGCTGC-3′	A4A5
5	5′-CAAACATTATGAGTAGTACAACACAAGCCGCAGAGG-3′	A8A10
6	5′-CCAAACATTATGGCTAGTAG	Ains2
7	5′-CCAAACATTATGCATCACCATCACCATCACAGTAGCACTACTCAGGCCC-3′	His
9	5′-CACAGAATTCTGATTGGATCC-3′
10	5′-GTGTATAGATATGAAAGGTACCGTG-3′
11	5′-GCTCTAGACTACAATGCTGTGTCCGCGCC-3′	Δ3
12	5′-GCTCTAGATCACAACAATGCGGTGTCCG-3′	Δ2
13	5′-GCTCTAGACTATCTCAACAATGCGGTGTC-3′	Δ1
14	5′-CGTCTAGATTAGATTCTCAACAATGCGG-3′	T228I
15	5′-CGTCTAGATTAGATTCTCAACAATGCGG-3′	T228L
16	5′-GCTCTAGATTAGACTCTCAACAATGCGG-3′	T228V
17	5′-GCTCTAGATTAGTTTCTCAACAATGCGG-3′	T228N
18	5′-GCTCTAGATTAGATATTATTTCTCAACAATGCGG-3′	OC
19	5′-CGTCTAGATCCGGTTCTCAACAATG-3′	+5
20	5′-GCTCTAGATTAGGTTGCCAACAATGCGGTG-3′	R227A
21	5′-TTAGGTGTCCGCGCCACTCGGT-3′	Δ5
22	5′-CTATTTGTTTGAGGGCAGTCGG-3′	Δ11
23	5′-TTAATTTCCGACCTTGGACTTC-3′	Δ18
24	5′-GTCCAAGGTAGGAAACGGCCGACTGC-3′	Y211G
PM149	5′-GATTACCATACACTAACA-3′
LK-10	5′-CAACAATGCGGTGTCCGCGCCAC-3′
LK-24	5′-CGCATTGTTGAGA(GATC)T(GC)TAATCTAAAC-3′	T228M, T228I, T228L, T228F, T228V
LK-26	5′-GATATGAAGGGTACCATGTATGTTAGGCCG-3′
LK-27	5′-CATCCTTAAAGTTTAGATTAGATATTATTTCTC-3′	BCV
LK-28	5′-TAATCTAAACTTTAAGGATGTCTTTTGTTC-3′	BCV
LK-29	5′-GCGATTATTTGGCCCACGGG-3′
LK-30	5′-CACCGCATTGTTGAGA(AT)A(GT)TAATCTAAACTTTAAGG-3′	T228Y
LK-31	5′-CACCGCATTGTTGAGAAATTAATCTAAACTTTAAGG-3′	T228N
LK-32	5′-CACCGCATTGTTGAGATAGTAATCTAAACTTTAAGG-3′	Δ1
LK-38	5′-ACCGCATTGTTGTAGACCTAATCTAAAC-3′	Δ2
LK-39	5′-AGATTAGGTCTACAACAATGCGGTGTCCGCGCC-3′	Δ2
LK-40	5′-GACACCTAGTAGTTGAGAACCTAATCTAAAC-3′	Δ5
LK-41	5′-GGTTCTCAACTACTAGGTGTCCGCGCCACTCGG-3′	Δ5
LK-42	5′-GGAAATTAATAGCGACTGCCCTCAAACAAACCG-3′	Δ18
LK-43	5′-GGGCAGTCGCTATTAATTTCCGACCTTGGACTTC-3′	Δ18

^aPrimers 1 to 24 were used for construction of expression vectors for VLP mutants. Primers PM149 to LK-43 were used for construction of transcription vectors for the generation of viral mutants.