FIG. 1.

HLA restriction and fine specificity of HIV-1 Rev-specific CTL clone TCC108. (A) Autologous and partially HLA class I-matched B-LCL cells were infected with rVV-rev (filled bars) or rVV-control (open bars) and analyzed for recognition by TCC108 cells in a standard chromium release assay (upper panel). Additional B-LCL cells were analyzed in a separate assay to confirm HLA-B14 restriction (lower panel). (B to D) Peptide-pulsed autologous B-LCL cells were analyzed for recognition by TCC108 cells in standard chromium release assays. Chromium-labelled target cells were incubated overnight with one of the 11 20-mer peptides together spanning the entire Rev sequence (B) or for 1 h with the N- and C-terminally truncated peptides before the addition of effector cells (C and D, respectively). Effector-to-target ratios were between 3:1 and 10:1 in all assays. The average percent specific lysis (with standard error) for triplicates is shown. Results similar to those presented in panels A and B were obtained with the Rev-specific CTL clones TCC102, TCC104, TCC106, and TCC110 (data not shown). The non-Rev-specific clone TCC112 did not lyse any of the rVV-infected or peptide-pulsed target cells (data not shown).