FIG. 5.

Specific inhibition of CF1-induced syncytium production in 293T and HeLa cells by FIV-infected domestic-cat plasma. Dilutions of either FIV-positive (+) (squares and circles) or FIV-negative (−) (diamonds and triangles) plasma specimens were added to cells at the time of transfection in 12-well plates (1 μg of DNA/well) and again with a change of medium 14 h later. Syncytia were scored at 48 h (as foci per well containing at least eight nuclei) by crystal violet staining of methanol-fixed cells; values are means ± standard deviations of data from three experiments.