TABLE 1.
Summary of syncytium induction by FIV envelope glycoprotein in human, feline, and rodent cell linesa
| Cell line | Syncytium induction resulting fromb:
|
||
|---|---|---|---|
| Transfection of CF1 or CT5 | Infection with CT5Δpol(VSV-G) | Coculture with 3201-FIV cells | |
| U87MG | None | None | None |
| SK-N-MC | None | None | None |
| 3T3 | None | None | None |
| 208F | None | None | None |
| CrFK | + | + | + |
| HOS | + | ND | None |
| CHO | None | ND | None |
| U87MG.CXCR4 | ++++ | ++++ | ++++ |
| SK-N-MC.CXCR4 | ++++ | ++++ | ++++ |
| 3T3.CXCR4 | ++++ | ND | ++++ |
| 208F.CXCR4 | ++ | ND | ++ |
| CrFK.CXCR4 | ++++ | ++++ | ++++ |
| HOS.CXCR4 | ++++ | ND | ++++ |
| HeLa | ++++ | ++++ | ++++ |
| 293 | ++++ | ND | ++++ |
| 293T | ++++ | ND | ++++ |
| Molt4 clone 8 | ND | ND | ++++ |
| SupT1 | ND | ND | ++++ |
| H9 | ND | ND | ++++ |
a
See Fig. 4 for representative photographs and text for quantitative scoring of syncytia, details of transfection, infection with replication-defective CT5Δpol(VSV-G), and number of experiments performed. Transfection efficiency controls are described in Materials and Methods.
b
++++, syncytia too numerous to count, or lytic destruction of monolayer; ++, >5 syncytia containing more than 8 nuclei each per cm2; +, <5 such syncytia per cm2; None, no synctia seen; ND, not done.