. 2021 Feb 19;2(9-10):427–439. doi: 10.1002/ansa.202000166

TABLE 3.

Optimized chromatographic condition

Method	Mobile Phase	Gradient(time vs. % of mobile phase B)	Column oven temp. (˚C)	Flow rate(mL/min)
Gradient	A: 0.1% HCOOH in water	0‐05, 15‐40, 35‐95, 40‐95, 42‐05, 45‐05	50	0.6
Gradient	B: 0.1% HCOOH in acetonitrile	0‐05, 15‐40, 35‐95, 40‐95, 42‐05, 45‐05	50	0.6

Column: Zorbax C18 (4.6 × 150 mm, 3.5 µm).