TABLE 4.

Validation details of studied corticoids by the developed method

No	Corticoids	RT	RRT	Recovery (%)	LOD (ng/ml)	LOQ (ng/ml)	Accuracy (%)	Intra‐dayprecision RSD (%)	Inter‐dayprecision RSD (%)	Correlation coefficient	Matrix effect (%)
	d4‐hydrocortisone	18.23	Ref. Std.
1	Triamcinolone	12.82	0.703	89	0.01	0.1	98.2	2.8	3.1	0.999	‐6.0
2	Fluocinolone	13.84	0.760	87	0.01	0.1	96.8	1.6	2.2	0.998	‐2.5
3	Prednisone	16.40	0.900	91	0.05	0.5	100.5	2.2	2.5	0.999	‐8.1
4	Isoflupredone	16.61	0.911	95	0.02	0.2	102.2	2.8	3.3	0.999	+12.0
5	Fluprednisolone‐6α	17.35	0.952	88	0.01	0.1	97.7	4.0	4.8	0.999	‐6.7
6	Fludrocortisone	17.60	0.966	94	0.02	0.2	99.1	1.9	2.1	0.998	‐7.0
7	Prednisolone	17.97	0.987	96	0.02	0.2	98.3	4.5	4.1	0.998	‐5.8
8	Flumetasone	18.04	0.990	78	0.02	0.2	101.2	3.3	3.5	0.998	+1.8
9	Prednisolone hemisuccinate	18.13	0.993	93	0.05	0.5	99.2	2.9	2.8	0.998	+4.5
10	d4‐hydrocortisone	18.20	1.000	88	0.05	0.5	100.5	4.1	3.8	1.000	‐3.4
11	Hydrocortisone	18.30	1.004	Not validated
12	Dexamethasone	18.60	1.021	95	0.01	0.1	100.2	2.7	3.5	0.999	+3.1
13	Diflorasone	18.75	1.027	87	0.01	0.1	98.9	0.9	1.2	0.999	‐6.1
14	Betamethasone	19.35	1.061	94	0.01	0.1	101.5	3.9	3.2	0.999	‐2.7
15	Paramethasone	19.59	1.075	92	0.01	0.1	97.9	2.7	3.8	0.998	‐4.2
16	Fluocinolone acetonide	19.75	1.084	83	0.01	0.1	99.0	3.5	4.7	0.998	+6.5
17	Methyl prednisolone	20.04	1.098	90	0.02	0.2	101.3	2.1	3.4	0.999	‐1.2
18	Triamcinolone acetonide	20.22	1.107	87	0.05	0.5	98.0	2.7	4.0	0.998	‐4.0
19	Beclomethasone	20.32	1.114	92	0.01	0.1	100.9	4.9	4.3	0.999	‐4.4
20	Cortisone	20.60	1.129	86	0.01	0.1	98.6	4.9	4.1	0.998	‐3.8
21	Flunisolide	21.31	1.169	90	0.02	0.2	99.1	2.7	3.6	0.998	+10.1
22	Isoflupredone acetate	21.45	1.176	79	0.05	0.5	99.8	3.2	2.9	0.999	+3.5
23	Desonide	22.04	1.210	84	0.05	0.5	102.0	1.8	2.0	1.000	‐12.6
24	Fluorometholone	22.20	1.217	88	0.02	0.2	100.8	3.5	4.8	0.999	‐6.2
25	Flurandrenolide	22.33	1.225	83	0.05	0.5	100.1	3.8	4.0	0.998	‐4.9
26	Prednisolone acetate	22.56	1.238	87	0.05	0.5	99.0	3.2	3.9	0.999	‐3.7
27	Deflazacort	22.90	1.256	Not validated
28	Meprednisone	23.08	1.266	Not validated
29	Paramethasone acetate	23.50	1.288	89	0.05	0.5	99.8	2.9	4.6	0.999	+1.2
30	Ulobetasol	23.85	1.309	92	0.05	0.5	98.5	3.1	4.0	0.998	‐8.9
31	Desoximetasone	24.00	1.317	83	0.05	0.5	97.8	3.7	5.2	0.999	‐3.1
32	Fluocinolone acetonide acetate	24.18	1.326	88	0.05	0.5	103.1	5.1	4.8	0.998	+2.8
33	Mometasone	24.85	1.364	93	0.05	0.5	102.8	2.9	4.1	0.998	‐11.0
34	Dichlorisone	25.05	1.375	92	0.05	0.5	101.2	4.8	5.3	0.999	‐8.9
35	Budesonide R or S	25.70	1.411	Not validated
36	Budesonide S or R	26.20	1.437	Not validated
37	Flurandrenolide acetate	26.30	1.442	85	0.05	0.5	97.9	3.9	6.1	0.999	‐6.5
38	Halcinonide	26.95	1.481	91	0.05	0.5	98.8	4.7	7.0	0.998	+5.2
39	Fluticasone propionate	27.17	1.490	94	0.01	0.1	103.0	6.2	5.5	0.998	+3.2
40	Amcinonide	27.30	1.498	86	0.01	0.1	100.7	3.2	4.8	0.999	‐4.1
41	Triamcinolone hexacetonide	28.70	1.570	92	0.02	0.2	97.7	5.5	6.8	1.000	‐3.7
42	Diflucortolone ‐21‐ valerate	29.00	1.591	92	0.05	0.5	102.3	2.7	4.3	0.999	‐4.9
43	Levonorgestrel or dextronorgestrel	30.00	1.644	Not validated
44	Dextronorgestrel or levonorgestrel	30.40	1.666	Not validated
45	Medrysone	31.50	1.730	90	0.01	0.1	101.0	6.1	7.2	0.998	+3.1
46	Deoxycorticosterone acetate	34.70	1.904	88	0.02	0.2	99.3	3.5	4.0	0.999	‐1.9